The pMIR-REPORT miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression.
- Clone miRNA targets and evaluate miRNA regulation
- Screen putative miRNA target sequences
- Includes both reporter and control vectors
System Basics
This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with a miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning of miRNA targets and evaluation of miRNA regulation. A second vector, pMIR-REPORT Beta-galactosidase Reporter Control Vector, is provided for normalization of transfection efficiency.
Applications
pMIR-REPORT Luciferase is designed for the cloning and testing of putative miRNA binding sites. pMIR-REPORT Luciferase can transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR miRNA Molecules or Anti-miR miRNA Inhibitor Molecules respectively. pMIR-REPORT Luciferase can also be used as a sequence screening tool to identify miRNA targets or screen libraries of Pre-miR miRNA Molecules to identify genes that regulate expression.
Product Format
The pMIR-REPORT miRNA Expression Reporter Vector System includes two reporter vectors (pMIR-REPORT Luciferase miRNA Expression Reporter Vector and pMIR-REPORT Beta-gal Reporter Control Vector) as supercoiled plasmids pre-transformed into E. coli and supplied as a glycerol stock. pMIR-REPORT Luciferase utilizes the powerful CMV promoter to drive high-level expression of firefly luciferase in mammalian cells. A miRNA target multiple cloning site follows the luciferase gene and is itself followed by a SV40 polyA region. The vector utilizes puromycin for selection in cell culture and a ColE1 Ori/Ampicillin-resistance gene for maintenance in E. coli. The pMIR-REPORT Beta-gal Control Vector is similar to pMIR-REPORT Luciferase, but has a beta-gal rather than a luciferase expression reporter and is intended for use in the normalization of transfection efficiency.
View pMIR-REPORT miRNA Expression Reporter Vector System on website