Quick (5 minute) method for cleaning and concentrating RNA. Fast-Spin column technology allows RNA to be eluted into minimal volumes (> 6 µl). Eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation. RNA Clean & Concentrator’-5 provides a simple and reliable method for the rapid preparation of up to ~5 µg (per prep.) of high-quality RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: just add the buffer to your sample, adjust the conditions for binding by adding ethanol, and the cleaned RNA is then concentrated into over 6 µl of RNase-free water using a Zymo-Spin’ IC Column. RNA fragments (over 17 bases) can be safely treated and recovered using this kit. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc. The entire procedure typically takes about 5 minutes.
View RNA Clean & Concentrator™-5 on Zymo Research’s website- RNA Clean & Concentrator™-5