Quick (20 minute) high-throughput (96-well) method for cleaning and concentrating RNA. Fast-Spin plate technology allows RNA to be eluted into minimal volumes (>10 µl). Eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation.
The ZR-96 RNA Clean & Concentrator provides a simple and reliable method for large-scale (96-well) purification and concentration of up to ~25 µg (per well) of high-quality RT-PCR-ready RNA. The procedure is based on the use of a unique single-buffer system and Fast-Spin plate technology. The procedure features a single-buffer system that allows for efficient RNA adsorption from up to 96 samples onto the matrices of the supplied Zymo-Spin I-96 Plate. The RNA is washed twice then eluted and concentrated into over 10 µl of RNase-free water.
The kit allows for small RNA recovery or removal by alternating the first two steps of the purification procedure. RNA fragments (over 17 bases) can be safely treated and recovered using this kit. The result is highly-concentrated purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc. The entire RNA isolation procedure typically takes about 20 minutes.View ZR-96 RNA Clean & Concentrator on Zymo Research’s website