ZR RNA MiniPrep

Quick (~15 minute) RNA isolation from a wide range of sources using Fast-Spin column technology. RNA eluted into volumes >25 µl is suitable for use in RT-PCR and other RNA-based procedures. Omits the use of organic denaturants, ß-mercaptoethanol and proteases. RNAlater compatible. The ZR RNA MiniPrep provides a quick method for high quality total RNA isolation from small amounts of cells and tissue. The ZR RNA MiniPrep isolates both large and small RNA species without the use of phenol or reducing agents. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol – no extra steps are required! RNA (~25 µg) from 10² to 10⁷ cells can be eluted into volumes as little as 25 µl in less than 15 minutes. The ZR RNA MiniPrep can be used to isolate total RNA from hard-to-lyse samples with the RNA Lysis Buffer and the ultra-high density ZR BashingBeads coupled with high-speed cell disrupters (e.g., FastPrep®-24) or to purify RNA directly from mixed DNA/RNA samples (e.g., in vitro transcription/translation).