Abstract: A variety of epigenetic modifications including post-translational modifications (PTMs) on histone tails regulate gene expression and have profound effects on cell signaling and human disease. N-terminal histone tails can have a variety of modifications, such as phosphorylation, methylation and acetylation at specific amino acid residues which are conserved throughout eukaryotes and function by altering chromatin structure and creating binding sites for chromatin readers, writers or erasers. Traditionally histone PTM levels are measured by western blot or ELISA, but these require larger amounts of material and can only look at one modification at a time.
Active Motif’s Histone H3 PTM Multiplex Assay is a high throughput bead-based ELISA assay designed for use with the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments to enable simultaneous interrogation of the levels of multiple histone H3 PTMs within the same sample. The assay is ideally suited for high-throughput screening and profiling of histone modification levels in limited and/or compound-treated samples. The multiplexing ability means you can simultaneously screen for on-target and off-target effects within the same sample. This Luminex technology offers several advantages over traditional methods (e.g. Western Blotting) including faster turnaround times and the ability to multiplex and utilize smaller sample amounts.
This webinar will include:
• An overview of the assay and the Luminex xMAP technology and instrumentation that is the basis of the assay
• Data comparing assay performance with traditional Western blotting
The Histone H3 PTM Multiplex Assay is the newest addition to Active Motif’s repertoire of Epigenetic Services.