OneStep qMethyl™ Kits enable easy, bisulfite-free DNA methylation analysis of a particular locus by combining Methylation Sensitive Restriction Enzyme digestion and real-time quantitative PCR into one step. Now the methylation masters at Zymo Research have added even more flavor to the qMethyl™ entrée with the launch of OneStep qMethyl™ Arrays, pre-designed assays for select targets served hot and ready to go on a 96-well plate that make rapid DNA methylation validation a standard menu item.
Supersize Me(thylation Screens)
OneStep qMethyl™ Arrays allow researchers to find key epigenetic biomarkers by screening large numbers of samples quickly and accurately, and avoiding troublesome bisulfite reactions too. Right now, single locus arrays are available for five human tumor suppressor genes RASSF1, RARB, CDKN2A, MGMT, CCND2 with additional panels for developmental biology, stem cells, neurological disorders, and other diseases on the way.
qMethyl™ Arrays: Ready for Action.
Yeah, qMethyl™ Arrays are a simple 1-step procedure for DNA methylation biomarker screening, validation & assessment, but check out these gems that are also part of the package:
- No primer design or complicated set up.
- Error free DNA methylation detection from a method with no loss of DNA.
- Pre-loaded primers and enzymes virtually eliminate pipetting errors.
- Shorter processing time of less than 5 hours start to finish while other profiling methods take over 8 hours.
- All reagents needed for the methylation analysis are included. Just bring your own sample.
qMethyl™ Arrays comes in a real-time PCR plate, pre-loaded for Single-Locus Methylation Detection, and all set up with Test and Reference reaction mixes, plus primers already aliquoted into the wells. The only thing left to do is add your sample DNA, seal, and load the plate into a thermocycler.
OneStep. Many Benefits.
If you’re ready to try out the full OneStep qMethyl™ Array experience first hand, head to the Zymo Research website for more info.