Sure genome-wide DNA methylation studies steal a lot of the limelight, but often it’s targeted DNA methylation studies that handle all of that much-needed data validation, or those deeper dives into key methylated regions.
That’s where targeted bisulfite sequencing shines. When you combine the reigning champ of DNA methylation analysis with next-generation sequencing, you get a pretty ideal tool for targeted DNA methylation analysis.
There’s just one problem. Who wants to handle all of that primer design, amplification, library prep, and other soul shattering bench work?
MethylCheck™ DNA Methylation Validation Services
Lucky for you, the savvy services team up at Zymo Research have put all of that DNA methylation experience to use, combining their well-established bisulfite conversion technologies with the power of next-generation sequencing to provide the most comprehensive, unique, and locus-specific DNA methylation analysis service available. If sitting back while the team handles your dirty work isn’t compelling enough, here’s more detail on what you can expect.
Cover All the Bases, Lots of Times
By harnessing the power of next-generation sequencing, each DNA methylation site of interest receives high sequencing depth of coverage for accurate, quantitative, and single-base resolution data output.
Zymo Research routinely sees sequencing depths >1000x assuring ample coverage of gene loci across all samples making detection possible down to very low methylation levels.
Throughput? You Bet
By incorporating the Fluidigm Access Array™ microfluidics PCR amplification instrument into this service platform, Zymo Research is able to simultaneously analyze dozens or even hundreds of samples in a short period of time.
But Wait, There’s Less
Compared with other targeted bisulfite sequencing methods, Zymo Research’s workflow uses relatively small amounts of bisulfite DNA as input, making it practical for most clinical samples. It’s also capable of sensitive detection of CpG methylation at very low methylation levels, making it an ideal platform for DNA methylation biomarker validation.
Inside the MethylCheck™ Targeted Bisulfite Sequencing Workflow
New to services? Concerned about dropping some precious sample into the mail? Here’s a breakdown of how it works.
- First, your samples are checked to confirm the quality and concentration and then converted using the EZ DNA Methylation-Lightning Kit.
- The flux capacitor is then set for maximum travel speeds (optional and highly variable).
- Next, post-bisulfite PCR primers for each target region are designed using a proprietary program Bisulfite Primer Seeker, and then experimentally validated using a standard genomic DNA control. Only primers which pass the validation with flying colors are used for the subsequent target amplification.
- After that, they perform the target amplification using Fluidigm Access Array 48.48 which allows parallel PCR reactions for 48 samples by 48 single-plex assays, then barcode for sequencing.
The workflow is simple and straightforward, but there’s only one way to know for sure, so give it a try, or get a quote today.