ChIP-IT® FFPE II

Perform ChIP on FFPE samples

Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for epigenetics techniques such as ChIP. Active Motif has leveraged its epigenetics expertise to develop commercially available kits to enable ChIP from FFPE tissue for use in Next Generation Sequencing (ChIP-Seq). The FFPE ChIP assay comes in two modules, the Chromatin Preparation Kit (for which there are two options, depending on sample quality) and the ChIP-IT® FFPE II Kit (to perform the ChIP-based enrichment), to allow flexibility dependent on type and amount of starting material

FFPE samples are typically limited in size, lack consistency in the methods used for formalin fixation and often display degradation and loss of antigenicity due to harsh fixation conditions or prolonged storage. These factors can affect the ease of chromatin preparation and may require a certain degree of optimization. The ChIP-IT® FFPE Chromatin Preparation Kits contain specially formulated reagents and protocol guidelines to extract high quality chromatin from histological slides or tissue sections. The ChIP-IT® FFPE Chromatin Preparation Kit is optimized for extracting chromatin from precious samples of unknown or poor quality. The ChIP-IT® FFPE Chromatin Preparation II Kit has a faster workflow optimized for high quality or freshly prepared FFPE samples.