Product Overview: Methylation of histone H3 at lysine 4 (H3 (methyl K4)) plays an important role in epigenetic regulation of genes. It has been well demonstrated that H3 (methyl-K4) leads to chromatin gene activation in many organisms. H3 (di-methyl K4) may serve as global epigenertic mark in euchromatin. Thus, identification of genes activated through H3 (methyl-K4) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.
EpiSeeker ChIP Kit – Histone H3 (methyl K4) (ab117143) enables the user to investigate in vivo H3 (methyl K4)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
ab117143 uses an antibody that detects Histone H3 di-methylated on K9.
If you are using tissue as starting sample material, we recommend the use of EpiSeeker ChIP Kit – Histone H3 (methyl K4) – Tissue (ab117145)
View EpiSeeker ChIP Kit – Histone H3 (methyl K4) on Abcam’s website