PNA Nanoparticles Offer Gene Editing Alternative, Reveal SCF As Editing EnhancerNovember 16, 2016CRISPR/Cas9 may be the flashiest gene editing tool out there right now, but there are certainly other contenders for that throne. We previously profiled Argonautes as one alternative; another is peptide nucleic acids, or PNAs. Like DNA, PNAs are strings of standard nucleotide bases, but instead of a charged phosphate backbone, they are held together […]
The Delicate Balance Between H3K27me2 and H3K27me3 Decides Stem Cell FateNovember 15, 2016The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27me) via its catalytic subunit Ezh2. While H3K27me3 and H3K27me1 have been associated with gene repression and activation respectively, the exact role of H3K27me2 remains under-explored. However, recent works in temperature-sensitive drosophila and in PRC2 knock out embryonic stem cells (ESCs) suggest a role […]
A CRISPR-Cas9 Pipeline for Efficiently Editing Blood Stem CellsNovember 3, 2016When it comes to gene therapy, blood diseases are prime candidates. Blood cells are constantly turning over, so you can quickly fix the blood supply if you replace the blood-producing stem cells in bone marrow; i.e., hematopoietic stem/progenitor cells (HSPCs). Transplanting marrow from a healthy donor is one approach, but a match can be difficult […]
Reporter of Genomic Methylation Delivers Imprinting and Epigenome Editing InsightsOctober 3, 2016Here at EpiGenie we like to think of ourselves as reporters of genomic methylation, but two new studies from the lab of Rudolf Jaenisch at MIT give us a run for our money and demonstrate the utility of their reporter of genomic methylation (RGM). Using RGM, the talented group brings forth a deeper understanding of […]
Proteomic Analysis of Aging MSCs Reveals New Targets to Enhance Musculoskeletal TherapiesSeptember 27, 2016Mesenchymal stem cells (MSCs) are surely the “workhorses” of both the body and the regenerative medicine field. They display multiple advantageous characteristics, including the ability to differentiate into musculoskeletal tissues, that make MSCs the go-to cell type for tissue-engineering solutions for disease/age-related disorders. Furthermore, many tissues require the proper on-going function of tissue-resident MSCs to […]
Colon Organoid Model: A New Tool for Cancer ResearchJuly 23, 2016The English sea captain and politician Sir Francis Drake believed in “sic parvis magna”, or “greatness from small beginnings”. While he hasn’t been around to see the recent explosion in organoid (or mini-organ) research, we are sure he would be impressed. Measuring a few millimeters in diameter and produced via three-dimensional differentiation of stem cells, […]
Top Upcoming Stem Cell ConferencesJuly 13, 2016Conferences are ideal places to hear insights from the leaders in your field, to watch the up-and-comers present their new and exciting visions, and to network with potential collaborators (i.e., drink and eat and have some fun!). To assist you with these endeavors, EpiGenie brings you the pick of the bunch: some of the most interesting stem […]
The Mixed Messages of Bivalent ChromatinJune 14, 2016Navigating the epigenetic landscape is no easy feat. Clear markings and smooth rides through its canals can quickly give way to a hectic hodgepodge of mixed signals and uncertain routes. A great example is bivalent chromatin, which contains marks of both activation and repression. Characterized by Bernstein et al. in 2006, the most studied bivalent […]
Self-Organizing Human Embryos Tell All from the Petri DishMay 26, 2016Humanity’s ongoing quest for knowledge has revealed unseen realms and unimaginable places, but there are still a few things that we know little about – the inner workings of a black hole, the composition of McDonald’s special sauce, and what goes on in the early post-implantation human embryo. There are good reasons for that latter […]
Improving Regenerative Therapies with Naked Mole Rat iPSCsMay 20, 2016At first glance the naked mole rat (NMR), or Heterocephalus glaber to his Latin speaking friends, might not win you over with its looks, but it truly is an amazing animal. It’s obviously comfortable in its own skin, but it also thrives in harsh environments, lacks pain sensitivity in its skin, has low metabolic and […]