The concept of chromatin contact mapping, or determining the three-dimensional structure conformation and interactions of chromatin domains, is now a reality because of Chromatin Conformation Capture (3C) and subsequent methods born out of that approach. Several new 3C-based techniques have emerged, each with particular strengths and applications, but the sheer variety creates a challenge when selecting the best method for a specific situation.
This guide from the good folks at Abcam will summarize current 3C methods, help you to choose the best option, and provide expert tips on optimizing the process in your lab. The guide covers:
- Emergence of Chromatin Conformation Capture (3C)
- Circularized Chromosome Conformation Capture (4C)
- Carbon Copy Chromosome Confromation Capture (5C)
- Chromatin interaction analysis by paired-end tag sequencing (CHIA-PET)
- Selecting a 3C method
Check out the complete 3C guide at the Abcam website.
For additional reading about chromosome conformation capture methods, check out this article on Hi-C and related methods from our friends at Active Motif.