Histone Deactylase (HDAC) researchers have been clamoring for more sensitive and reliable HDAC assays in order to generate the high quality data that previous techniques don’t always deliver. The HDAC-Glo™ I/II Assays and Screening Systems from Promega aim to shine new light on the matter with highly sensitive assays that ditch the old, troublesome false positive and negatives with optimized substrates and Ultra-Glo™ luminescent reagents.
Simply Sensitive HDAC Measurement
HDAC-Glo™Assays are powerful in their simplicity. The process is much like standard fluorescence-based assays, only anywhere from 10 to 100 times more sensitive. Promega describes it as “a single-reagent-addition, homogeneous, add-mix-measure protocol for easy implementation from benchtop to screening.” Sounds pretty straightforward to us!
Fast-Track to HDAC data
Typical HDAC-Glo™ reactions take just 15-45 minutes, and aside from the setup, most of that time is hands-off. In addition, a luminescent signal with more than a 3hr half-life means that the HDAC-Glo™ platform is easily adapted to most benchtop luminometers or even automated, and high-throughput systems.
Linear assays mean you’ll get straight-shooting results with type I and II HDACs.
One HDAC assay system, many sample options
HDAC-Glo™ Assays allows you the flexibility to query a whole host of sample types. Try it with viable cells, extracts or purified recombinant enzymes.
Make it Glo
The HDAC-Glo™ I/II Assays and Screening Systems are perfect for any number of HDAC research applications, including:
• Determining the potency of HDAC inhibitors.
• Using purified enzymes to profile HDAC inhibitors.
• Multiplexing cell viability and HDAC potency assays together in the same well.
• Confirm deacetylase inhibition with control substrates.
Luckily, you can achieve this Glo without the use of bronzers or tanning beds. Just head over to the Promega website to learn more, and get your HDAC-Glo™ I/II Assays and Screening System today!