Histone purification system for high throughput applications Active Motif’s Histone Purification Microplate Kit enables purification of core histones (H2A, H2B, H3 and H4) using very small amounts (as little as 0.9 x 105 cells) of starting material, and its microplate spin column format enables elution into a 96-well plate to easily transition into downstream high-throughput […]
- Chromatin
ChIP-IT® PBMC Kit
Perform ChIP on difficult-to-lyse PBMCs: Active Motif’s ChIP-IT® PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in […]
ChIP-IT® FFPE Chromatin Preparation Kit
Perform ChIP from FFPE samples Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for techniques such as ChIP. Active Motif has leveraged its ChIP expertise to develop the first commercially available kits to enable ChIP from FFPE tissue for use in Next Generation […]
HDAC-Glo™ Class IIa Assays
The HDAC-Glo™ Class IIa Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class IIa from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled […]
HDAC-Glo™ 2 Assays
The HDAC-Glo™ 2 Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class I enzyme 2 from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a […]