Perform ChIP from FFPE samples
Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. However, working with FFPE samples presents a challenge for techniques such as ChIP. Active Motif has leveraged its ChIP expertise to develop the first commercially available kits to enable ChIP from FFPE tissue for use in Next Generation sequencing. The FFPE ChIP assay comes in two modules, the ChIP-IT® FFPE Chromatin Preparation Kit and ChIP-IT® FFPE Kit, to allow flexibility dependent on type and amount of starting material.
FFPE samples are typically limited in size, lack consistency in the methods used for formalin fixation and often display degradation and loss of antigenicity due to harsh fixation conditions or prolonged storage. These factors can affect the ease of chromatin preparation and may require a certain degree of optimization. The ChIP-IT® FFPE Chromatin Preparation Kit contains specially formulated reagents and protocol guidelines to extract high quality chromatin from histological slides or tissue sections.
The ChIP-IT® FFPE Kit contains all the necessary reagents and protocols to allow enrichment of both high abundance targets, such as histones, or low abundance proteins, such as transcription factors from the limited amounts of chromatin obtained from FFPE samples. Antibody and PCR primer controls are also provided in this kit to evaluate the successful execution of the ChIP procedure.View ChIP-IT® FFPE Chromatin Preparation Kit on Active Motif’s website