Human recombinant LSD1 expressed in E. Coli LSD1 catalyzes the oxidative demethylation of mono- and dimethylated H3K4, producing hydrogen peroxide and formaldehyde in the process. H3K4 methylation is considered a transcription-activating chromatin mark, and in vivo LSD1 is frequently found in association with the transcriptional corepressor protein CoREST and HDACs 1 or 2. LSD1 is […]
- Histone Demethylase Kits and Assays
EpiSeeker Histone H3 (K4) Demethylase Activity Quantification Assay Kit
Product Overview: Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3(K4) demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3(K4), and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a […]
EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit
Product Overview: KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation. EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) allows the user to measure inhibition of KDM1/LSD1. The kit […]
EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit
Product Overview: KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation. EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The […]
EpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay Kit
Product Overview: Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. H3(K9) demethylation is regulated by at least two classes of demethylases, JMJD1 (which can remove mono- and di-methylation from H3(K9)) and JMJD2 (which can remove trimethylation […]