Product Overview: Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. H3(K9) demethylation is regulated by at least two classes of demethylases, JMJD1 (which can remove mono- and di-methylation from H3(K9)) and JMJD2 (which can remove trimethylation from H3(K9)).
EpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458) allows the user to measure activity/inhibition of H3(K9) specific histone demethylases using cell/tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3(K9) specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
View EpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay Kit on Abcam’s website