Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event […]

ools for Measuring Levels of Silencing-associated CpG Methlyation in DNA at Specific Genes Features Detect definitive changes in the CpG methylation pattern of genomic DNA Correlate transcriptional regulation with changes in methylation pattern Investigate epigenetic mechanisms of developmental control and disease progression Advantages Flexibility of experiment with a universal method for any gene of interest […]