ools for Measuring Levels of Silencing-associated CpG Methlyation in DNA at Specific Genes
Features
- Detect definitive changes in the CpG methylation pattern of genomic DNA
- Correlate transcriptional regulation with changes in methylation pattern
- Investigate epigenetic mechanisms of developmental control and disease progression
Advantages
- Flexibility of experiment with a universal method for any gene of interest
- Sensitive detection allows use of as little as 1 ng of DNA
- Easy to use with no restriction digests or Southern blots required
The CpGenome and CpG WIZ Systems allow rapid and simple detection of the methylation status of a gene using methylation-specific PCR (MSP). This advanced technique enables the precise mapping of methylation patterns in the CpG islands of genomic DNA. The CpGenome DNA Modification Kits employ an initial bisulfite reaction to modify the DNA, followed by gene-specific PCR amplification with primers designed to distinguish methylated from unmethylated DNA. The CpG WIZ gene specific amplification kits include all necessary primers and reagents for MSP analysis of several critical genes known to exhibit differential methylation.
View CpG WIZ® Amplification Kits on Millipore’s website