The CpGenome™ Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice and enzymatically methylated at all CpGs by M.SssI methyltransferase 1. It can be used as a positive control for gene methylation studies, and is fully compatible with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847) and CpG WIZ® methylation-specific amplification systems.
One vial containing 5 µg (20 µl) of CpGenome™ Universal Methylated Mouse DNA Standard at a concentration of 250 ng/µL.
Methylation-specific PCR (MSP) was performed on the CpGenome™ Universal Methylated Mouse DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® Oct-4 kits (Catalog No. S7840) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for this Standard.View CpGenome™ Universal Methylated Mouse DNA Standard on Millipore’s website