Product Overview: The position of the histone H3 at lysine 9 (H3 (K9)) is important for epigenetic regulation of genes, as it can be either methylated or acetylated. It has been demonstrated that switching H3(acetyl K9) to methylation on H3 (methyl K9) leads to chromatin gene silencing in many organisms. Thus, identification of genes silenced through H3 (methyl K9) is of particular significance for understanding and analyzing biological processes under normal and pathological conditions.
EpiSeeker ChIP Kit – Histone H3 (methyl K9)-Tissue (ab117144) enables the user to investigate in vivo H3 (methyl K9)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
ab117144 uses an antibody that detects Histone H3 di-methylated on K9.
If you are using cells (adherent or suspension) as starting material, we recommend the use of EpiSeeker ChIP Kit – Histone H3 (methyl K9) (ab117141)
View EpiSeeker ChIP Kit – Histone H3 (methyl K9) – Tissue on Abcam’s website