Our High-Specificity miRNA QRT-PCR Detection kits provide qualified reagents for the polyadenylation of microRNAs (miRNAs) from total RNA and synthesis of first-strand cDNA from the tailed miRNAs as well as a novel PCR enzyme formulation and QPCR detection reagents designed to give the utmost specificity for miRNA QPCR detection.
The kit includes 2 modules consisting of: polyadenylation of the miRNA at the end and reverse transcription to convert the polyadenylated miRNA to cDNA (miRNA 1 st Strand cDNA Synthesis Kit) and cDNA detection by QPCR (High-Specificity miRNA QPCR Core Kit). Our High-Specificity miRNA QRT-PCR detection kit offers extreme specificity and sensitivity. Our kit can preferentially detect mature miRNA from as little as 15 ng of total RNA input on a variety of sample types. Our kit detects miRNA directly in lysed cells or in total RNA isolated from tissues, cell cultures, and FFPE tissues.
- High-Specificity miRNA qRT-PCR Detection Kit