We have validated several well-known miRNA primer sequences that may prove as useful tools for monitoring patterns of miRNA expression in certain cancers. Using a novel primer design strategy we optimized primers for different human miRNA that were selected based upon their roles in cancer and development. The miRNAs were validated using 15-250 ng input total RNA and resulted in a linear signal when quantitating miRNA of high, medium, and low abundance. These forward primers can be used in combination with the universal reverse primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work.
Validated Highly Specific Forward Primers
- Detects mature miRNA
- Detects human, mouse, rat, worm and other species
- Highly-specific sequences assure single nucleotide discrimination
- High-Specificity miRNA QRT-PCR Detection Kit
- microRNA Sequence Information