The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Sigma offers a complete solution for DNA methylation analysis with the Imprint DNA Modification Kit. This product contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples.
Benefits Include:
- Fast – procedure can be completed in less than 2 hours compared to overnight methods.
- Unparalleled Sensitivity – only 50 pg or 20 cells required for modification.
- Complete Conversion – greater than 99% conversion of unmethylated cytosines.
- Robust – more than 50% DNA recovery through treatment and purification.