The Methylated-DNA IP Kit utilizes IP technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. The kit features a highly specific anti-5-methylcytosine monoclonal antibody for the “capture” and separation of methylated DNA from non-methylated DNA in only a few hours. Typically, over a hundred-fold enrichment of methylated DNA vs. non-methylated DNA can be achieved with the use of this kit. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including: PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing and microarray.
Application: The Methylated-DNA IP Kit utilizes IP technology for the enrichment of 5 methylcytosine-containing DNA with a highly specific anti-5-methylcytosine monoclonal antibody for the “capture” and separation of methylated DNA from non- methylated DNA in only a few hours, from any pool of fragmented genomic DNA for use in genome-wide methylation analysis.
Components: The Methylated-DNA IP Kit comes as a two component kit: the kit box along with a separate icebox containing the cold-storage components.
Enrichment Factor: > 100 fold for Methylated versus Non-methylated DNA. Elution: Ultra pure, enriched methylated DNA can be eluted with 10 µl Elution Buffer or water.
View Methylated-DNA IP Kit on Zymo Research’s website