Exiqon’s ISO 9001:2000 certified microRNA profiling service covers everything from an initial consultation to the final data analysis. The service is carried out with automated hybridization stations in state of the art ozone-free laboratories using our latest generation of miRCURY LNA microRNA microarray products. Your results will be presented in an easy-to-read report within 2-4 weeks.
When you place an order with Exiqon’s microRNA Profiling Services, you are assured direct communication with the scientists performing your experiments throughout the duration of the project. Each service project begins with a free consultation with an expert who has a wealth of experience in microRNA expression profiling. At this consultation, the optimal experimental design and final data analysis is discussed and agreed upon.
Preferred experimental setup
It is crucial for the success of the profiling study that the chosen experimental setup is designed for your specific needs, and we put a lot of effort into finding the optimal design. Based on the number and type of comparisons you wish to make, we will offer advice on whether to choose a common reference, universal reference or direct comparison setup.
Samples
For good results, it is very important to use high quality RNA samples. At the initial consultation we will offer advice on methods of purification and the number of biological replicas needed. If possible, it is therefore ideal if the consultation takes place before you prepare your RNA samples.
Choice of arrays
You will be given the choice between the two available types of miRCURY LNA microRNA Arrays (version 11.0 of our human, mouse and rat array or version 11.0 of our other species array). Our array platform is the most sensitive platform on the market. Due to the incorporation of LNA in the capture probes, more than 90% of our probes have a detection limit of less than 10 amol. This enables us to perform microRNA profiles with very low levels of total RNA input. All our capture probes are Tm-normalized to ensure uniform and high quality performance across the array. Read more about the miRCURY LNA microRNA Arrays
Data analysis
Our profiling service includes a data analysis summary report which is described in detail in the Report section of the workflow above. At the initial consultation we will discuss how the data analysis is optimized in relation to the chosen experimental setup.
View MicroRNA Array Services on Exiqon’s website