microRNA-specific primers for first-strand cDNA synthesis and highly sensitive real-time PCR of mature microRNAs.
- Sensitivity – Start microRNA quantitation from just 10 pg total RNA
- Dynamic range – Accurate quantitation over a range of 8 logs
- Specificity – Specific discrimination of closely related microRNAs and between mature and precursor microRNAs
- Reproducibility – Highly reproducible data with over 98% correlation between experiments
- Fast and reliable – Easy two-step protocol takes less than 3 hours
- Validated microRNA assays – Fully validated, high-quality LNA-based microRNA-specific primer sets
Features
The miRCURY LNA microRNA PCR System enables extremely rapid, accurate and sensitive quantitation of mature microRNAs annotated in miRBase. The system combines the unique advantages of LNA technology and the well-known SYBR Green detection technology in a simple two-step procedure designed for total RNA samples.
First, the microRNA in the total RNA sample is converted to cDNA by reverse transcription using a sensitive, thermostable reverse transcriptase and a microRNA-specific primer. Then, the cDNA is amplified by real-time PCR. The use of LNA nucleosides makes it possible to build microRNA-specific primers in both reaction steps, thereby enabling highly specific targeting of the mature microRNA of interest.
Coverage
A variety of high-quality microRNA primer sets are available. To find a particular primer set, please use the search function below. You can search by entering a microRNA sequence, product name or number. For advanced searches, see the search tips below.
All microRNA primer sets are validated for optimal performance ensuring low background and high specificity and sensitivity. Newly validated microRNA primer sets are added continuously.
Note
The Universal PCR primer for the real-time PCR step of the protocol is supplied with the miRCURY LNA SYBR Green Master Mix.
In addition to this product, a miRCURY LNA microRNA real-time PCR assay also requires the following reagents:
- miRCURY LNA First-strand cDNA Kit
- miRCURY LNA SYBR Green Master Mix