The Agilent miRNA Complete Labeling and Hyb Kit is for use with the Agilent miRNA Microarray System and provides a novel Cyanine 3-nucleotide along with array labeling and hybridization reagents. In combination with the miRNA microarray probe design, the Cyanine 3-Cytidine bisphosphate (pCp) reagent selectively labels and hybridizes mature miRNAs. Use of these reagents ensures consistent results.
- 100 ng total RNA input per labeling reaction
- Sufficient reagents to label and hybridize 24 microarrays (3, 8-plex miRNA microarray slides are contained within one kit)
- Hybridization time is 20 hours
- Contents include all enzymes and buffers, including Cyanine 3-pCp for labeling and Hi-RPM buffer necessary to perfom assay
- Hybridization kits chould be stored at room temperature
- All reagents should be stored at -20ºC