Monitor for sensitivity and accuracy
The Agilent One-Color RNA Spike-In Kit contains in vitro synthesized, polyadenylated transcripts. When hybridized onto a microarray, the user can use the expected versus observed log ratios to monitor a microarray workflow for linearity, sensitivity and accuracy. This kit is a required component of the Agilent One-Color Gene Expression platform.
Feature:
- Sample required per reaction is 100 – >500 ng total RNA
- Each transcript was constructed by the cloning of a unique 55-mer sequence into the human adenovirus type 6 E1A 13S gene
- Transcripts specifically anneal only to complementary microarray probes with minimal self- or cross-hybridization
- Spike-In transcripts specifically anneal only to complementary control probes on Agilent’s microarrays, with minimal self- or cross-hybridization
- Dynamic range is approximately 6 orders of magnitude
- Contents include spike mix and dilution buffer
- Kit should be stored initially at 80°C for up to one year from date of result. Diluted spike mixes can be stored at 20°C for up 2 months.