Epigenetic modifications are regarded as fundamental to the regulation of gene expression. DNA methylation is one such modification that plays crucial roles in widespread biological phenomena including the regulation of gene activity, gene imprinting, embryonic development and X-chromosome inactivation in higher organisms.
Current methods used to evaluate DNA methylation including bisulfite sequencing, Methylation Specific PCR (MSP), HPLC and Methylated DNA Immunoprecipitation (MeDIP) have proven too costly, time consuming, or inappropriate for the investigation of large numbers of loci. OneStep qMethyl™ from Zymo Research provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment. Simply add DNA into the appropriate well then quantitate via real-time PCR…OneStep!
- Premade 96-well assay for bisulfite-free determination of DNA methylation status.
- Ideal for rapid high-throughput screening of single-locus DNA methylation levels.
- Processing time only ~4 hrs