EZ Nucleosomal DNA Prep Kit

The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and micrococcal nuclease.

Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’- phosphate and 3’-hydroxyl termini. Atlantis dsDNase digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven Fast-Spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes.

  • Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
  • Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
  • High quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR amplification, arrays, Southern blot analysis, DNA quantification, sequencing, and other molecular applications.
View EZ Nucleosomal DNA Prep Kit on Zymo Research’s website

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