The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and micrococcal nuclease. Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’- phosphate and 3’-hydroxyl termini. Atlantis dsDNase digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven Fast-Spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes.
- Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
- Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
- High quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR amplification, arrays, Southern blot analysis, DNA quantification, sequencing, and other molecular applications.