No Epigenetic Modification is an Island: How Reader Proteins Functionally Interpret Complex Chromatin StatesMarch 25, 2024Like no man is an island and instead relies on those surrounding them, single epigenetic modifications generally do not function in solitary. Now, an absorbing new study from a synergistic epigenomics squad reports how epigenetic reader proteins functionally interpret chromatin states made up of complex combinations of disparate epigenetic modifications. A highly collaborative team guided […]
reChIP Doubles Down on Low-input Profiling of Bivalent ChromatinMarch 5, 2024Sometimes, when you’re dealt the right hand, doubling down is a sound strategy. Although it can be a risky move in blackjack, a new ChIP-seq method makes it a sure thing when you’re dealing with bivalent chromatin. The strategy, which includes important preparation steps and two rounds of chromatin immunoprecipitation (ChIP), provides sensitive genome-wide profiles […]
FORGEdb Deciphers Genetic Variants and Disease Mechanisms to Tell Whether Your Gene Regulation Mechanism is a Touchdown or a Field Goal!February 26, 2024Performing experiments to understand gene regulation and do not want to miss any extra points? Well, FORGEdb may be the method for you! Building on previous research, this new method can help scientists unravel the mysteries behind complex diseases by identifying candidate functional variants and uncovering target genes and mechanisms. Developed by a team led […]
SIMPLE-seq Collaborates Chemistries to Report Single-Cell 5mC and 5hmCFebruary 20, 2024Collaborations work best when team members’ abilities complement each other. That’s what makes SIMPLE-seq work so well—it’s a simple, easy combination of two methods that complement each other to provide both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) datasets in one experiment in single cells and molecules. Continuing on the recent theme of methods that report both 5mC […]
Relax and Let NanoSPA Analyze m6A and Ψ RNA Mods for YouFebruary 20, 2024We could all use a spa day once in a while, so light some lavender-scented candles, take a deep breath, and let NanoSPA analyze the RNA modifications in your samples. This new nanopore sequencing analysis pipeline provides data on both N6-methyladenosine (m6A) and pseudouridine (Ψ) modifications at the same time, reducing stress and saving time. Instead […]
TIME-Seq Flies When Constructing Cost-Effective Epigenetic ClocksFebruary 19, 2024If time drags and money disappears when looking at DNA methylation-based epigenetic clocks, you are “in time” to hear of a newly described efficient and cost-effective bisulfite sequencing approach! Time flies – but money does not – with “tagmentation-based indexing for methylation sequencing” or TIME-seq! An on-time team led by David A. Sinclair (Harvard Medical […]
eBook: New & Novel Methods for Epigenetic AnalysisFebruary 7, 2024Epigenetics is not new, but methods used for analysis certainly can be! In this eBook, we take a look at, and present in a simple format, some of the hottest new methods for researchers looking at DNA Methylation, RNA modifications, Chromatin and Gene Regulation. Topics Include: Differential Methylation Data Are Quick, Easy, and Inexpensive with […]
GEMS Makes the m6A RNA Modification Shine in Living CellsFebruary 5, 2024Unlike DNA or histone methylation, knowledge about N6-methyladenosine (m6A) modification of RNA is a little cloudy. It’s involved in some cancers and other diseases, but we don’t know as much about its expression in living cells. Now, a new method called GEMS makes m6A shine bright like a diamond for drug discovery and delivery of […]
Bilingual Proteins Establish and Maintain Heritable Silent Chromatin DomainsFebruary 5, 2024Whether you learn through hitting the books or traveling the globe – speaking two languages has many advantages. Now, a study into the basis of gene expression regulation reports how bilingual “read-write” proteins that “speak” two epigenetic languages can help us to learn about the complex mechanisms controlling sequence-independent heritable gene silencing. The dynamic duo […]
uCoTarget Is a Single-Cell Multiomics Method for Multiple Histone ModificationsJanuary 17, 2024Everyone loves a bargain, and a new method called CoTarget really delivers. It’s a method that delivers single-cell multiomics data on multiple histone modifications and transcription factors, as well as the transcriptome. It can easily scale up to analyze a million single cells, and unlike similar methods, it doesn’t require microfluidic devices or special transposon […]