This assay is a simple two-step procedure performed in a microtiter plate. In the first step, samples are incubated with the HDAC assay substrate, allowing deacetylation of the substrate. Next, the Activator Solution releases p-nitroanilide from the deacetylated substrate or standard.
- Histone Deacetylase
HDAC Assay Kit, fluorometric detection 384 well format
The 384 well format kit is a newer version of the tried and true non-radiographic HDAC assay. As in the previous kit, samples are first incubated with the HDAC Assay substrate, allowing deacetylation of the fluorometric substrate. After incubation, the activator solution is added, and releases a fluorophore from the deacetylated substrate or standard. The […]
HDAC Assay Kit, fluorometric detection
This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.
HDAC Assay Buffer, 5X
Designed for use in the Histone Deacetylase Assay (HDAC) Kit (cat#’s 17-320, 17-372, and 17-374)
SIRTainty™ Class III HDAC Assay
The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC […]