The HDAC-Glo™ Class IIa Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class IIa from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled […]
- Histone Deacetylase
HDAC-Glo™ 2 Assays
The HDAC-Glo™ 2 Assays are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class I enzyme 2 from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a […]
HDAC3/NCOR1 complex human recombinant
Human recombinant HDAC3/NCOR1 produced in insect cells Background Information: Unlike other class I HDAC enzymes, HDAC3 is found in co-repressor complexes with the SMRT/N-CoR proteins. HDAC3 contains a unique domain structure which includes both nuclear localization and nuclear export sequences. HDAC3 has been implicated in cancer, as HDAC3 inhibitors produce anti-proliferative effects in various cancer […]
HDAC6 (His-tag) human recombinant
Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells Background Information: Histone deacetylase 6 (HDAC6) is member of the class II HDAC family, shich share significant sequence homology to the yeast HDA1 protein. HDAC6 is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic […]
Histone Deacetylase Radiometric (3H) Assay Kit
This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the […]