Whereas a large body of evidence exists regarding the existing form of methylation, very little is yet known about 5-hydroxymethylcytosine methylation. 5-methylcytosine is converted into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases, but little is known about the role of 5-hydroxymethylcytosine in the cell. Many tools exist to study 5-methylcytosine, but few are able […]
- 5-hmC Detection
5-hmC Detection
5-Hydroxymethylcytosine, or 5-hmC is a modified form of 5-mC. It has been shown that 5-hmC and the broadly studied 5-mC cannot be distinguished by sodium bisulfite conversion.
DNA Degradase Plus™
DNA Degradase Plus™ from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus™ is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than […]
5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set
The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three DNA standards that are linear dsDNA, 897 bp, and have the same sequence. The only difference is that each contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines (see figure). Since the sequence and extent of cytosine modification is known, this DNA standard set […]
T4 Phage B-glucosyltransferase (T4-BGT)
T4 Phage β-glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.
Quest 5-hmC Detection Kit™
The Quest 5-hmC Detection Kit™ from Zymo Research allows for sequence specific detection of 5-hydroxymethylcytosine within DNA using a simple and efficient reaction setup. Utilizing a robust and highly specific 5-hmC Glucosyltransferase enzyme, 5-hydroxymethylcytosine in DNA is specifically tagged with a glucose moiety yielding a modified base, glucosyl-5-hydroxymethylcytosine. Product Highlights: Method to specifically distinguish 5-hydroxymethylcytosine […]