Product overview: MBD2 (methyl-CpG binding domain protein 2) belongs to a family of MBD domain containing proteins. This family of proteins associates with heterochromatin by their interaction with methylated DNA at CpG islands, and appear to ‘read’ and ‘translate’ the DNA methylation signal into transcriptional repression by recruiting silencing complexes and histone deacetylases among other […]
- DNA Methylation Enrichment Kits
UnMethylCollector
UnMethylCollector is a novel technology that allows for positive identification ofunmethylated DNA fragments. The kit specifically enriches for unmethylated CpG islands using a CXXC binding domain from mouse Mbd1. UnMethylCollector allows researchers to identify hypomethylated promoters from normal or diseased samples. This simple and sensitive technique provides DNA suitable for use in many downstream applications, such as real time […]
MethylMagnet Proteins
MethylMagnet proteins are versatile tools for the study of CpG methylation in DNA. These proteins contain the methyl binding domain (MBD) of the mouse MBD2 protein fused to the glutathione-S-transferase protein (GST) from S japonicum. The two domains are separated by a linker containing a thrombin cleavage site. The MBD from the MBD2b protein was […]
MethylMagnet® mCpG DNA Isolation Kit
The MethylMagnet mCpG DNA Isolation Kit is used to isolate methylated DNA from a genomic sample. The MethylMagnet kit utilizes the MethylMagnet GST-MBD fusion protein and glutathione magnetic beads for capture of methylated DNA. It can be used to isolate mCpG DNA from 1 ng to 1 µg of genomic DNA, or it can be […]
Methylated-DNA IP Kit
The Methylated-DNA IP Kit utilizes IP technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. The kit features a highly specific anti-5-methylcytosine monoclonal antibody for the “capture” and separation of methylated DNA from non-methylated DNA in only a few hours. Typically, over a hundred-fold […]