Our High-Specificity miRNA QRT-PCR Detection kits provide qualified reagents for the polyadenylation of microRNAs (miRNAs) from total RNA and synthesis of first-strand cDNA from the tailed miRNAs as well as a novel PCR enzyme formulation and QPCR detection reagents designed to give the utmost specificity for miRNA QPCR detection. The kit includes 2 modules consisting of: polyadenylation […]
- miRNA qRT-PCR
Cells-to-Cts + QuantiMir™ complete Kit
Efficient, Sensitive and Accurate Highly efficient poly(A) tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of microRNAs. The optimized reaction conditions and buffer components maximize cDNA yield using anywhere from several micrograms to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables the ease […]
miScript SYBR Green PCR Kit
The miScript SYBR Green PCR Kit is based on the proven technology of QuantiTect SYBR Green Kits. The miScript SYBR Green PCR Kit includes QuantiTect SYBR Green PCR Master Mix and the miScript Universal Primer which is specific for the universal tag sequence on the 5′ end of the cDNA. For quantification of miRNA Sensitive and […]
miScript Primer Assay
miScript Primer Assays are miRNA-specific primers used in combination with the miScript SYBR Green PCR Kit for real-time PCR of miRNA using SYBR Green detection. The miScript SYBR Green PCR Kit includes the miScript Universal Primer which is necessary for successful amplification. For quantification of miRNA Sensitive and specific detection and quantification of miRNA Fast quantification […]
miScript PCR Controls
miScript PCR Controls allow normalization of real-time PCR results in miRNA quantification in human, mouse, and rat. Features: Effective in human, mouse, and rat High amplification efficiencies of close to 100% Constant expression levels in a variety of cells and tissues Easy to order via GeneGlobe For accurate and reproducible results in miRNA quantification by real-time […]