The miScript SYBR Green PCR Kit is based on the proven technology of QuantiTect SYBR Green Kits. The miScript SYBR Green PCR Kit includes QuantiTect SYBR Green PCR Master Mix and the miScript Universal Primer which is specific for the universal tag sequence on the 5′ end of the cDNA.
For quantification of miRNA
- Sensitive and specific detection and quantification of miRNA
- Fast quantification of multiple miRNAs from a single cDNA
- Quantification of miRNA and mRNA from the same cDNA
- Flexible plate formats and scales for miRNA-specific assays
- Custom assay design for miRNAs not listed in miRBase
The miScript PCR System comprises the following components:
- miScript Reverse Transcription Kit for simple, single-step cDNA synthesis.
- miScript SYBR Green PCR Kit that includes the miScript Universal Primer which allows detection of miRNAs in combination with an miRNA-specific primer.
- miScript Primer Assay, an miRNA-specific forward primer which is used in combination with the miScript SYBR Green PCR Kit.
The cDNA serves as the template for real-time PCR analysis using the miScript Primer Assay in combination with the miScript SYBR Green PCR Kit. miRNAs are amplified using the miScript Universal Primer together with the miRNA-specific primer (the miScript Primer Assay).
A single cDNA synthesis reaction is sufficient to interrogate hundreds of miRNAs by real-time PCR using different miScript Primer Assays, allowing comprehensive expression profiling of known miRNAs . The cDNA can also be used for detection of mRNA using gene-specific primer pairs, allowing simultaneous detection of reference genes, such as GAPDH, or any other mRNAs of interest, such as the mRNA targeted by a particular miRNA. QIAGEN offers QuantiTect Primer Assays for real-time PCR detection of mRNA in combination with QuantiTect SYBR Green PCR Kits or with QuantiTect SYBR Green PCR Master Mix from the miScript SYBR Green PCR Kit.