miScript Primer Assays are miRNA-specific primers used in combination with the miScript SYBR Green PCR Kit for real-time PCR of miRNA using SYBR Green detection. The miScript SYBR Green PCR Kit includes the miScript Universal Primer which is necessary for successful amplification.
For quantification of miRNA
- Sensitive and specific detection and quantification of miRNA
- Fast quantification of multiple miRNAs from a single cDNA
- Quantification of miRNA and mRNA from the same cDNA
- Flexible plate formats and scales for miRNA-specific assays
- Custom assay design for miRNAs not listed in miRBase
The miScript PCR System comprises the following components:
- miScript Reverse Transcription Kit for simple, single-step cDNA synthesis.
- miScript SYBR Green PCR Kit that includes the miScript Universal Primer which allows detection of miRNAs in combination with an miRNA-specific primer.
- miScript Primer Assay, an miRNA-specific forward primer which is used in combination with the miScript SYBR Green PCR Kit.
miScript Primer Assays are miRNA-specific primers used in combination with the miScript SYBR Green PCR Kit for real-time PCR of miRNA using SYBR Green detection. The miScript SYBR Green PCR Kit includes the miScript Universal Primer which is necessary for successful amplification.
miScript Primer Assays are available in single tubes and can be ordered online from the GeneGlobe Web portal. miScript Primer Assays are available for all human, mouse, rat, and virus miRNAs listed in the current version of miRBase, and are continually updated to ensure that you are working with the most up-to-date sequence. For newly discovered human, mouse, rat, or virus miRNAs which are not listed in miRBase, miScript Primer Assays can be custom designed.
miScript Primer Assay Sets and Plates
Human, Mouse, or Rat miScript Assay Sets are available in 96-well or 384-well plates. These sets provide assays for >95% of the miRNAs listed in miRBase version 13.0. The Human miScript Assay Set provides 846 miScript Primer Assays for human miRNAs. The Mouse miScript Assay Set provides 639 miScript Primer Assays for mouse miRNAs. The Rat miScript Assay Set provides 343 miScript Primer Assays for rat miRNAs. To facilitate experimental design and data analysis, every set includes 6 miScript PCR Controls on each plate (Hs_RNU1A_1, Hs_RNU5A_1, Hs_RNU6B_2, Hs_SNORD25_1, Hs_SCARNA17_1, and Hs_SNORA73A_1;and 2 blank wells on each plate.
For flexible screening, predesigned and custom (not listed in miRBase) miScript Primer Assays of choice can be ordered in 96-well or 384-well plates by simply uploading an Excel sheet of the desired plate layout.
View miScript Primer Assay on QIAGEN’s website