This assay is a simple two-step procedure performed in a microtiter plate. In the first step, samples are incubated with the HDAC assay substrate, allowing deacetylation of the substrate. Next, the Activator Solution releases p-nitroanilide from the deacetylated substrate or standard.
- Millipore
HDAC Assay Kit, fluorometric detection 384 well format
The 384 well format kit is a newer version of the tried and true non-radiographic HDAC assay. As in the previous kit, samples are first incubated with the HDAC Assay substrate, allowing deacetylation of the fluorometric substrate. After incubation, the activator solution is added, and releases a fluorophore from the deacetylated substrate or standard. The […]
HDAC Assay Kit, fluorometric detection
This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.
HDAC Assay Buffer, 5X
Designed for use in the Histone Deacetylase Assay (HDAC) Kit (cat#’s 17-320, 17-372, and 17-374)
p300/CBP Radiometric (3H) Assay Kit
Histone Acetyltransferase Assay (HAT) kit containing nonradioactive components for assessing the histone acetyl transferase (HAT) activity of immunoprecipitated p300/CBP.