Specific glycosylation of 5-hydroxymethylcytosine (5-hmC) 5-hmC Glucosyltransferase catalyzes the transfer of a glucose moiety from uridine diphosphoglucose (UDPG) to existing 5-hmC in DNA to form glucosyl-5-hydroxymethylcytosine (glucosyl-5-hmC). 5-hmC Glucosyltransferase does not modify cytosines or methylated cytosines (5-mC). Glucosylation of 5-hmC can be used for sequence-specific, locus-specific, or global quantification of 5-hmC. Validation: 5-hmC Glucosyltransferase was […]
- Millipore
CpGenome™ Human Methylated & Non-Methylated DNA Standard Set
CpGenome™ Human Methylated & Non-methylated DNA Standards are purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. The CpGenome™ Human Methylated DNA Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase 2. These standards are […]
CpGenome™ 5-mC and 5-hmC DNA Standard Set
CpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. Materials Provided: Three vials, each containing 2 µg (40 µL) […]
AbSurance™ Complete Core Histone Antibody Specificity Array
The AbSurance Complete Core Histone Antibody Specificity Array is comprised of 89 high quality modified and unmodified histone peptides ( >95 % purity) spotted on Immobilon® P PVDF membranes in a 96-well format. One membrane contains 46 histone H3 peptides (8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified). The other membrane contains 43 histone […]
EZ-Magna ChIP™ HT96 Chromatin Immunoprecipitation Kit
The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative […]