EpiMark™ Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the […]
- New England Biolabs
EpiMark™ Bisulfite Conversion Kit
Each EpiMark Bisulfite Conversion Kit contains sufficient reagents to perform the bisulfite conversion reaction of 48 samples. For optimal results, 50 ng–2 μg DNA is recommended. Kit Components: Sodium metabisulfite (each aliquot is sufficient for 8 reactions) Solubilization Buffer 2 x 1 ml Desulphonation Reaction Buffer (concentrate) 3 ml EpiMark spin columns with 2 ml […]
T4 Phage B-glucosyltransferase (T4-BGT)
T4 Phage β-glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.
EpiMark™ 5-hmC and 5-mC Analysis Kit
New England Biolabs is pleased to introduce the EpiMark™ 5-hmC and 5-mC Analysis Kit, a simple and robust method for the identification and quantitation of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) within a specific DNA locus. This enzymatic approach utilizes the differential methylation sensitivity of the isoschizomers MspI and HpaII in a simple 3-step protocol (described […]