The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5’…CG…3′.
- New England Biolabs
HpaII
HpaII is a methylation sensitive restriction enzyme that recognizes CCGG. When used with the isoschizomer MspI, information on methylation status can be obtained.
DpnII
Methylation sensitive restriction enzyme the recognizes GATC. When used in conjunction with DpnI, information about methylation status can be obtained.
DpnI
Methylation sensitive restriction enzyme the recognizes GATC. When used in conjunction with DpnII, information about methylation status can be obtained.
MspJI
MspJI, an EpiMark™, validated product is a modification-dependent endonuclease that recognizes mCNNR sites and generates a double-stranded DNA break on the 3´ side of the modified cytosine at N9/N13. The recognized cytosine modifications include C5-methylation (5-mC) and C5-hydroxymethylation (5-hmC).