MspI is a restriction enzyme that recognizes CCGG. When used with the methylation sensitive isoschizomer HpaII, information on methylation status can be obtained.
MspI
DNA Methylation Dependent Restriction Enzymes - New England Biolabs
DNA Methylation Dependent Restriction Enzymes - New England Biolabs
MspI is a restriction enzyme that recognizes CCGG. When used with the methylation sensitive isoschizomer HpaII, information on methylation status can be obtained.
DNA Methylation Dependent Restriction Enzymes - New England Biolabs
McrBC is an endonuclease which cleaves DNA containing methylcytosine* on one or both strands. McrBC will not act upon unmethylated DNA. Sites on the DNA recognized by McrBC consist of two half-sites of the form (G/A)mC. These half-sites can be separated by up to 3 kb, but the optimal separation is 55-103 base pairs. McrBC […]
DNA Methylation Dependent Restriction Enzymes - New England Biolabs
LpnPI, an EpiMark™ validated product, is a modification-dependent endonuclease which recognizes CmCDG sites and generates a double-stranded DNA break on the 3´ side of the modified cytosine at N10/N14. Recognized cytosine modifications include C5-methylation (5-mC) and C5-hydroxymethylation (5-hmC) (1).
DNA Methylation Dependent Restriction Enzymes - New England Biolabs
FspEI, an EpiMark™ validated product, is a modification-dependent endonuclease which recognizes CmC sites and generates a double-stranded DNA break on the 3´ side of the modified cytosine at N12/N16. Recognized cytosine modifications include C5-methylation (5-mC) and C5-hydroxymethylation (5-hmC).
The EpiMark™ Methylated DNA Enrichment Kit will selectively bind and enrich double-stranded methyl-CpG DNA from fragmented genomic DNA with as little as 5 ng of input DNA. Methylated DNA is isolated from fragmented genomic DNA (5 ng–25 μ g) by binding to the methyl-CpG binding domain of human MBD2a protein fused to the Fc tail […]