Chromatrap® ChIP 96-well microplates for high throughput ChIP are based on the novel Chromatrap® spin-column ChIP technique, incorporating essentially 96 separate Chromatrap® spin-columns in one device. Designed to enable researchers to perform many ChIP experiments in parallel, assays are performed either by using hand-held multiple pipetting techniques or by using automated liquid handling robots for even higher throughputs.
The volume and dimensions of the wells in the 96-well microplate are very similar to that of the established Chromatrap® Spin-columns so the optimised spin-column protocol forms the basis of the approach to performing high throughput ChIP analysis in 96-well microplates.
Each well has its own porous disc at the bottom in which Protein A or Protein G has been covalently attached. The functionalised discs have the same chemistry and stoichiometry as the spin columns so all of the reagent volumes and timings can be directly inferred from the spin-tube protocol. The 96-well microplate is supplied with a close fitting collection plate to recover the retained chromatins at the elution step.
Protein A and Protein G variants of the Chromatrap® high-throughput 96-well microplate are currently being assessed at several beta sites using the existing spin-column protocol with the aim of developing an optimised high throughput protocol for broader use.
Interested researchers who would like to take part in the beta trials and protocol optimisation should get in touch with kevin.quinlan@chromatrap.com or contact Porvair Filtration Group on +44-1489-864330 / +1-804-550-1600 / email support@chromatrap.com.
