CpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications.
Three vials, each containing 2 µg (40 µL) of CpGenome™ Cytosine DNA Standard, CpGenome™ 5-mC DNA Standard, or CpGenome™ 5-hmC DNA Standard, at a concentration of 50 ng/μL.
The three Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.