The most comprehensive microRNA Precursor Clone Collection in HIV-based Lentiviral Vectors available anywhere.
There are expected to be over 1,000 microRNAs encoded in the human genome and they function by either blocking translation of, or degrading, mRNA species corresponding to specific genes. While the number of verified human microRNAs is expanding, there is an increasing need for effective functional testing. System Biosciences (SBI) is offering an extensive collection of microRNA precursors in lentiviral vectors that can be used to modulate the expression of their targeted mRNAs in vivo, and thereby study microRNA function. The constructs are built in a lentiviral vector backbone, so they can be packaged into lentiviral particles and delivered to primary cells, stem cells, or other hard-to-transfect cell lines.
Features and Benefits
- Pre-microRNA expression from constitutive CMV promoter
- Reliable delivery to dividing or non-dividing cells
- Monitor positive expressing cells with copGFP as a reporter under the control of a constitutive human elongation factor 1 (EF1) promoter, which is functional in most cell lines
- Convenient sorting of transfected or transduced cells
- Pool of prepackaged viral particles containing the entire microRNA Precursor Collection for high-throughput phenotypic screens also available