Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Features & Benefits:
• Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
• Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
• Includes spin columns to make DNA purification easier and more reliable – no more messy phenol-chloroform extractions
• Complete kit with all required reagents for reliable and reproducible results
• Compatible with ChIPAb+ validated antibody and primer sets