miR-SNaREs and PIWI-SNaREs – Small Non-coding RNA Enrichment
Overexpress Epitope tagged microRNA processing factors and PIWI factors for IP pull-downs of RNAs and associated protein complexes. Lentivector based expression of DGCR8 (Pasha), DICER and AGO2 (Argonaute) for miR-SNaREs and PIWIL-1,-2,-3 and -4 for PIWI-SNaREs. All constructs contain N-terminal HA tags for simple immunoprecipitation experiments. Constructs available with co-expression of either RFP for fluorescent cell sorting or Puro for selection of stable cell lines.
Features and Benefits
- Lentivector-based cDNA expression of microRNA processing factors or piRNA-binding(PIWI) factors
- Overexpressed protein factors contain N-terminal HA tag for immunopurification (IP pull-downs)
- Choose from either RFP or Puro markers.
- All expression constructs fully sequence-verified.
- The expression constructs are protein expression validated.
- Discover low abundance microRNAs or piRNAs.
- Identify new epigenetic RNAs and associated processing protein factors
- Enrich for RISC-associated messenger RNAs or active PIWI complex-associated RNAs.