Mir-X miRNA qRT-PCR SYBR Kits are complete, dual-function systems for performing first-strand cDNA synthesis and quantitative PCR (qPCR) to precisely measure the level of your favorite miRNAs. The kits are available in economical, large-sized formats that provide 200 or 600 qPCR reactions, and each kit includes a Mir-X miRNA First-Strand Synthesis Kit and SYBR Advantage qPCR Premix.
Simple and Sensitive
A simple, single-step reaction uses an optimized mix of poly(A) polymerase and SMART MMLV Reverse Transcriptase to synthesize first-strand cDNA from your RNA sample (Figure 1). The cDNA is then specifically amplified and quantified by qPCR using your miRNA-specific primer and our SYBR Advantage qPCR Premix. Multiple miRNA species, as well as the mRNA targets of the miRNAs, can be amplified from a single cDNA sample. The system is extremely sensitive and able to detect miRNAs down to 50 copies.
Highly specific Detection
To demonstrate the specificity of Mir-X miRNA quantification we used a series of 8 highly similar synthetic Let7 miRNA variants. We first spiked each of the Let7 miRNAs into separate samples of yeast poly(A) +RNA and generated cDNA using the Mir-X single-tube reaction. We then tested a panel of variant-specific primers with each cDNA sample to determine each primer’s ability to specifically and individually quantify the Let7 subtypes in the cDNA sample. Despite the high degrees of similarity among the variants and the primers, Mir-X qPCR was highly specific in detecting each Let7 variant.
View Mir-X™ miRNA qRT-PCR SYBR Kit on Clontech’s website