Gene editing with CRISPR/Cas9 is here, there, and everywhere. We have seen gene editing in human somatic cells, pluripotent stem cells, and even embryos. However, we should also be looking at CRISPR-based gene editing somewhere else: in a living, breathing human. The question is – where does the current technology stand on the in vivo […]
- "epigenetic editing"
Search Results for: epigenetic editing
Casilio: a CRISPR/Cas9 and Pumilio Hybrid for Epigenome Editing
Life is full of challenges, and when that challenge is epigenome editing even a rock star like CRISPR/Cas9 stands to benefit from helping a hand. Epigenome editing with CRISPR/Cas9 involves using a dead Cas9 (dCas9) that confers the precision of the genome editing system without nuclease activity. The standard approach involves utilizing a fusion protein […]
CRISPR Gene Editing Prefers the Naïve Side of Pluripotency
While many across the universe have been pondering over the decision between the light and the dark side of the Force, a more pressing decision confronts CRISPR-based gene editing in stem cells: the primed or the naïve side of pluripotency!? Pluripotent Stem Cells (PSCs), such as human induced pluripotent stem cells (hiPSCs), human embryonic stem […]
Validating GWAS with Epigenome Editing
GWASs (genome-wide association studies) have found a lot of genetic variants associated with various traits and diseases. But GWASs suffer from that old weakness, the mantra of every good scientist: correlation does not imply causation. The problem gets even worse when you find out most GWAS variants are in non-coding regions of DNA, meaning they […]
Epigenome and Transcriptome Editing
Genome editing refers to an emerging branch of biotechnology that is the realization of earlier genetic engineering technologies. Using these biotechnologies researchers are able to target specific DNA sequences and induce a double stranded break, taking advantage of recombination to create synthetic genetic content in a host genome. One of the way researchers are uncovering […]